User blog comment:J Shepard/M.E.2 Death's/@comment-1878375-20100210003012

I know you can kill Shepard before the final battle by romancing Mordin. Also, you can not acquire squad members by just going through the Omega 4 relay as soon as you have the IFF installed. Hmm, oh, and you can turn Legion over to Cerberus, which is as good as killing him. Other than these things, I've not seen a way to kill anyone else of or get rid of them before the suicide mission. Also, I still haven't found a way of killing Shepard outside of Mordin, or a way of killing ALL squad members. Some always survive for me =/